Proteomics raw data and analysis

Proteomics analysis of the peptides form control and Alzheimer's diseases brain extracts that bind to the W-Tau peptide by affinity chromatography.

The W-Tau peptide (sequence: KKVKGVGWVGCCPWVYGH), derived from Tau intron 12, was purchased from Abyntek Biopharma S.L. W-Tau peptide interaction with human brain extracts from cortical samples from patients with sporadic Alzheimer's disease and control individuals (HUB-IDIBELL Brain Bank, Spain) was analyzed using CNBr-activated Sepharose 4B affinity chromatography. Proteins were concentrated at the stacking/resolving gel interface of SDS-PAGE, digested with trypsin, and desalted using OMIX Pipette C18 tips (Agilent Technologies). Protein identification was performed via reverse-phase liquid chromatography coupled to tandem mass spectrometry (RP-LC-MS/MS) using an LTQ-Orbitrap-Velos-Pro (Thermo Scientific) under a dynamic exclusion setting. Peptide identification was conducted using PEAKS Studio XPro, searching against the UniProt database. Functional network analysis was performed using STRING.