Purified proteins were quantified using the Standard Test Tube Protocol (Pierce BCA Protein Assay Kit) following manufacturer specifications by producing a BSA colour response curve as standard curve. Protein samples were diluted when required in order to be within the assay sensitivity (0 - 2 mg/ml)
Funding
Royal Society Research Grant (RG130585) and BBSRC New Investigator Grant (BB/N005570/1)