Pollen stack

A sample of pollen, imaged with a Nikon A1 confocal microscope, with excitation wavelength 488 nm, 40x/1.2 water immersion objective and GaAsP detector. Due to custom intermediate optics on the illumination/detection path, the objective was underfilled, to produce an effective NA of approximately 0.5-0.6. Image size is 512x512x181 and voxel size 78x78x250 nm (x,y,z).