Normalized qRT-PCR data

qRT-PCR data which has been normalized to two housekeeping genes, Rpl19 and Rp49. We first used ANOVA to check for potential significant differences in the transcription of the housekeeping genes across experimental groups, and found there to be no significant differences.

All qRT-PCR reactions for each sample were run in triplicate on a 96-well plate, with each well containing 5 µL iTaq Universal SYBR Green Supermix (BioRad), 400 nM of forward and reverse primer, 3.2 µL H2O, and 1 µL cDNA (total volume = 10 µL). Reactions were run on a BioRad CFX Connect Real-Time system. After an initial melt step at 95˚C for 30 sec, targets were amplified using 40 cycles of 95˚C for 5 sec, and 60˚C for 30 sec. Following PCR amplification, a melt curve was run to ensure that a single product was produced. Primer sequences for all transcripts have been previously published and are available in the Methods section of the associated manuscript.