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New Potent Acetylcholinesterase Inhibitors in the Tetracyclic Triterpene Series

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posted on 2007-11-01, 00:00 authored by Thibault Sauvaître, Mireille Barlier, Denyse Herlem, Nohad Gresh, Angèle Chiaroni, Daniel Guenard, Catherine Guillou
A new highly selective inhibitor of acetylcholinesterase (AChE) was discovered by high-throughput screening. Compound 1 was synthesized from a natural product, the N-3-isobutyrylcycloxobuxidine-F 2. A new extraction protocol of this compound is described. The hemisynthesis and optimization of 1 are reported. The analogs of 1 were tested in vitro for the inhibition of both cholinesterases (AChE and BuChE). These compounds selectively inhibited AChE. Extensive molecular docking studies were performed with 2 and AChE employing Discover Biosym software to rationalize the binding interaction. The results suggested that ligand 2 binds simultaneously to both catalytic and peripheral sites of AChE.