figshare
Browse
DOCUMENT
S1 Fig.PDF (9.47 kB)
DOCUMENT
S2 Fig.PDF (11.17 kB)
IMAGE
S3 Fig.PNG (9.42 kB)
DOCUMENT
S4 Fig.PDF (39.87 kB)
IMAGE
S5 Fig.PNG (38.32 kB)
DATASET
S1 File.XLSX (130.23 kB)
TEXT
S2 File.TXT (7.91 MB)
TEXT
S3 File.TXT (8 MB)
TEXT
S4 File.TXT (2.74 MB)
TEXT
S5 File.TXT (7.91 MB)
TEXT
S6 File.TXT (8.01 MB)
TEXT
S7 File.TXT (2.74 MB)
DOCUMENT
S1 Text.DOCX (17.96 kB)
1/0
13 files

Nested Association Mapping of Stem Rust Resistance in Wheat Using Genotyping by Sequencing

dataset
posted on 2016-05-17, 21:12 authored by Prabin Bajgain, Matthew N. Rouse, Toi J. Tsilo, Godwin K. Macharia, Sridhar Bhavani, Yue Jin, James A. Anderson

We combined the recently developed genotyping by sequencing (GBS) method with joint mapping (also known as nested association mapping) to dissect and understand the genetic architecture controlling stem rust resistance in wheat (Triticum aestivum). Ten stem rust resistant wheat varieties were crossed to the susceptible line LMPG-6 to generate F6 recombinant inbred lines. The recombinant inbred line populations were phenotyped in Kenya, South Africa, and St. Paul, Minnesota, USA. By joint mapping of the 10 populations, we identified 59 minor and medium-effect QTL (explained phenotypic variance range of 1% – 20%) on 20 chromosomes that contributed towards adult plant resistance to North American Pgt races as well as the highly virulent Ug99 race group. Fifteen of the 59 QTL were detected in multiple environments. No epistatic relationship was detected among the QTL. While these numerous small- to medium-effect QTL are shared among the families, the founder parents were found to have different allelic effects for the QTL. Fourteen QTL identified by joint mapping were also detected in single-population mapping. As these QTL were mapped using SNP markers with known locations on the physical chromosomes, the genomic regions identified with QTL could be explored more in depth to discover candidate genes for stem rust resistance. The use of GBS-derived de novo SNPs in mapping resistance to stem rust shown in this study could be used as a model to conduct similar marker-trait association studies in other plant species.

History