figshare
Browse

Localization Precision Analysis Data for Interferometric Single-Molecule Localization Microscopy

dataset
posted on 2025-05-09, 06:21 authored by Lusheng GuLusheng Gu

This dataset contains measurement data of localization precision using single-molecule interferometric localization microscopy. The localization precision of the single-molecule interferometric localization microscope was measured using 40 nm fluorescent microspheres. This dataset includes raw image files, data result files processed with MATLAB, and result images. The results show localization precisions of 6.6 nm, 6.4 nm, and 4.7 nm for the X, Y, and Z directions respectively, achieving isotropic single-molecule localization precision and uniform resolution in all directions.

File description:

  • IntComp.mat: Brightness calibration data
  • pixComp.mat: Pixel calibration data
  • RegInfo.mat: Raw image cropping and transformation data
  • spool_A_1_MMStack_Default.ome.tif: Camera A data
  • spool_A_2_MMStack_Default.ome.tif: Camera B data
  • spool_A_1_MMStack_Default.ome_result.mat: Processed result data file
  • spool_A_1_MMStack_Default.ome_result_sel.png: Illustration of selected fluorescent microspheres
  • spool_A_1_MMStack_Default.ome_result1.png: Summary of processing results including XY localization error, brightness, phase error, modulation depth, etc.
  • spool_A_1_MMStack_Default.ome_result2.png: XYZ localization precision analysis result image

Funding

National Science and Technology Innovation 2030 Major Program Grand No. 2022ZD0211905 National Natural Science Foundation of China 32322050

History

Usage metrics

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC