LC-MS/MS setup for SCoPE2 experiments

LC-MS/MS setup for SCoPE2 experiments. The left panels show the typical gradient parameters used for SCoPE2 runs. Non-standard portions of the gradient include turning the voltage off during the initial phase to the gradient to reduce the contamination of the heated capillary. Material collected on the emitter tip is then removed with sheath-gas briefly directed at the tip through a blower elbow. After this point the voltage is applied and scan data collected. The right panels show the attachment of the blower elbow to a Q-Exactive classic instrument.