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Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition

posted on 2015-10-16, 03:44 authored by Giulia Fontemaggi, Teresa Bellissimo, Sara Donzelli, Ilaria Iosue, Barbara Benassi, Giorgio Bellotti, Giovanni Blandino, Francesco Fazi

Treatment of leukemia cells with 1,25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22–3p and let-7e-5p.