IS5 Element Characterization Across Pseudomonas syringae Strains

<p dir="ltr">Supplemental information for our manuscript concerning IS element distributions and IS5 element characterization across Pseudomonas syringae. This folder contains 3 files:</p><p><br></p><p dir="ltr">FileS1) an excel spreadsheet documenting IS5 locations in three complete P. syringae pv. lachrymans genomes. One sheet lists all of the IS5 elements that match by BlastP and the other sheet contains a rosetta stone whereby synteny of all IS5 elements across these three genomes was compared by hand</p><p dir="ltr">FileS2) a .fasta file containing the query sequence for blastP searches for InsH</p><p dir="ltr">FileS3) a genbank flat file (.gff) for the assembly of strain DBL328. This is a kanamycin resistant isolate of <i>P. syringae </i>pv. lachrymans 107 (MAFF301305) in which an IS5 element hopped in front of the <i>npt</i> gene thereby enabling expression and kanamycin resistance in this strain</p><p dir="ltr">FileS4) a .tsv file of the results of ISfinder characterization across all P. syringae complete genomes vetted as part of this manuscript</p><p dir="ltr">FileS5) a spreadsheet containing all <i>insH </i>DiamondBlastP hits from Pseudomonas.com as well as a summary worksheet of all complete genomes and numbers of IS5 elements</p><p dir="ltr">FileS6) a .csv file of all complete genomes used in the manuscript as well as Genbank accession numbers</p><p dir="ltr">FileS7) an .xls spreadsheet of annotated IS5 insertion sites in P. syringae pv. lachrymans genomes</p><p dir="ltr">FileS8) .zip file of data underlying RT-qPCR results</p><p dir="ltr">FigS1) a .svg heatmap resulting from FastANI comparisons across pv. lachrymans genomes at GTDB</p>