From Code to Life: Transforming Computer Algorithms into DNA

After obtaining the desired sequence, DNA synthesis is essential to produce the H-Bot in physical form. This process can be efficiently managed through a closed control loop system. The system comprises four vials containing dNTPs: deoxyadenosine triphosphate (dATP), deoxyguanosine triphosphate (dGTP), deoxythymidine triphosphate (dTTP), and deoxycytidine triphosphate (dCTP). Each vial is equipped with a micropipette for precise handling.

During synthesis, the first nitrogenous base displayed on screen triggers a robotic arm attached to a CDS micropipette to dispense a specified concentration of the corresponding dNTP. This process repeats until 23 separate DNA strands are synthesized. The resulting physical DNA strand is termed "Artificial Single-Stranded DNA" or "assDNA" strand for short. Read more ...

Garg, R. , Vyas, A. , Khan, A. , Tariq, M. (2024), 'Codes beyond Bits and Bytes: A Blueprint for Artificial Life', World Academy of Science, Engineering and Technology, Open Science Index 213, International Journal of Biotechnology and Bioengineering, 18(9), 114 - 126.