Flow cytometry raw data involving in the article "<b>Neuro-immune–related gene signatures define molecular subtypes and prognostic score in bladder cancer with SERPINE2 as a potential therapeutic target</b>"
<p dir="ltr"><b>Purpose</b></p><p dir="ltr"> This experiment aimed to determine the effect of SERPINE2 gene knockdown on apoptosis in T24 human bladder cancer cells using the Annexin V-FITC/PI dual staining method. </p><p dir="ltr"><b>Experimental Design</b></p><p dir="ltr"> Groups: Control (Vector), shRNA3, Cell line: T24 (human bladder carcinoma), Method: Flow cytometry (FITC/PI dual channel), Replicates: 3 biological repeats </p><p dir="ltr"><b>Materials</b></p><table><tr><td><p dir="ltr">Item</p></td><td><p dir="ltr">Brand</p></td><td><p dir="ltr">Catalog No.</p></td><td><p dir="ltr">Note</p></td></tr><tr><td><p dir="ltr">DMEM High Glucose Medium</p></td><td><p dir="ltr">Gibco</p></td><td><p>11965-092</p></td><td><p dir="ltr">with 10% FBS</p></td></tr><tr><td><p dir="ltr">1× PBS</p></td><td><p dir="ltr">YEASEN</p></td><td><p dir="ltr">41403ES76</p></td><td><p dir="ltr">pre-cooled</p></td></tr><tr><td><p dir="ltr">Trypsin-EDTA Solution</p></td><td><p dir="ltr">Gibco</p></td><td><p>25200-072</p></td><td><p>0.25%</p></td></tr><tr><td><p dir="ltr">Annexin V-FITC Apoptosis Detection Kit</p></td><td><p dir="ltr">Beyotime</p></td><td><p dir="ltr">C1062L</p></td><td><p dir="ltr">per manufacturer’s protocol</p></td></tr></table><p dir="ltr"><b>Instrumentation </b></p><p dir="ltr">Flow cytometer: BD FACSCanto II (BD Biosciences, USA) </p><p dir="ltr">Excitation laser: 488 nm </p><p dir="ltr">Channels: FITC: Ex/Em = 488/530 nm, PI: Ex/Em = 488/615 nm </p><p dir="ltr">Software: FlowJo v10.8 </p><p dir="ltr"><b>Data Description</b> </p><p dir="ltr">Format: .FCS </p><p dir="ltr">Each group includes 3 replicates </p><p dir="ltr">Example file names: T24-NC_1.fcs T24-shRNA3_1.fcs </p><p dir="ltr"><b>Summary of Results</b> </p><p dir="ltr">Knockdown of SERPINE2 significantly increased the apoptosis rate of T24 cells compared with the control group (<i>P</i> < 0.05).</p>
