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Figure 6 and 7 from manuscript Sparsely-Connected Autoencoder (SCA) for single cell RNAseq data mining

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posted on 26.08.2020, 14:36 authored by Raffaele CalogeroRaffaele Calogero
Dataset used to generate figure 6 and 7.

Figure 6: Analysis of human breast cancer (Block A Section 1), from 10XGenomics Visium Spatial Gene Expression 1.0.0. demonstration samples. A) SIMLR partitioning in 9 clusters (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/HBC_BAS1_expr-var-ann_matrix_Stability_Plot.pdf). B) Cell stability score plot for SIMLR clusters in A (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/HBC_BAS1_expr-var-ann_matrix_Stability_Plot.pdf. C) SIMLR clusters location in the tissue section (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/HBC_BAS1_expr-var-ann_matrix_spatial_Stability.pdf). D) Hematoxylin and eosin image (figure6and7/HBC_BAS1/spatial/V1_Breast_Cancer_Block_A_Section_1_image.tif).

Figure 6: Analysis of human breast cancer (Block A Section 1), from 10XGenomics Visium Spatial Gene Expression 1.0.0. demonstration samples. A) SIMLR partitioning in 9 clusters (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/HBC_BAS1_expr-var-ann_matrix_Stability_Plot.pdf). B) Cell stability score plot for SIMLR clusters in A (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/HBC_BAS1_expr-var-ann_matrix_Stability_Plot.pdf. C) SIMLR clusters location in the tissue section (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/HBC_BAS1_expr-var-ann_matrix_spatial_Stability.pdf). D) Hematoxylin and eosin image (figure6and7/HBC_BAS1/spatial/V1_Breast_Cancer_Block_A_Section_1_image.tif).

Figure 7: Information contents extracted by SCA analysis using a TF-based latent space. A) QCC (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/HBC_TF_SIMLRV2/9/HBC_BAS1_expr-var-ann_matrix_stabilityPlot.pdf). B) QCM (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/HBC_TF_SIMLRV2/9/HBC_BAS1_expr-var-ann_matrix_stabilityPlotUNBIAS.pdf). C) QCM/QCC plot, where only cluster 7 show, for the majority of the cells, both QCC and QCM greater than 0.5 (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/HBC_TF_SIMLRV2/9/HBC_BAS1_expr-var-ann_matrix_StabilitySignificativityJittered.pdf). D) COMET analysis of SCA latent space. SOX5 was detected as first top ranked gene specific for cluster 7, using as input for COMET the latent space frequency table (figure6and7/HBC_BAS1/Results_simlr/raw-counts/HBC_BAS1_expr-var-ann_matrix/9/outputvis/cluster_7_singleton/rank_1.png). Input counts table for SCA analysis is made by raw counts.


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