Fig. 5b.JPG

Fig. 5a:  Bacterial expression of phytase appA_S of Shigella sp. CD2 in E.coli BL21(DE3): SDS-PAGE analysis of recombinant phytase (rAppA_E). Lane, M- Molecular weight markers in kDa, 1- soluble fraction of induced BL21 (DE3) transformed with pET20b (+) vector, 2-pellet fraction of induced BL21 (DE3) transformed with pET20b (+) vector, 3-soluble fraction of induced BL21 (DE3) transformed with pET20b(+) -appA_S, 4- pellet fraction of induced BL21 (DE3) transformed with pET20b(+) -appA_S.