Methods, FASTA files and relevant statistics for draft MAGs generated from an anaerobic nitrate-reducing benzene-degrading enrichment culture

Presented within are draft metagenome assembled genomes (MAGs) created from multiple genome assemblies of nitrate-reducing benzene-degrading enrichment cultures (NRBC) derived from soil samples collected from a decommissioned gas station located in Toronto, Canada. The primary objective was to obtain closed genomes of organisms from NRBC in order to understand each organism's role in the enrichment culture. Two Microbial Resource Announcements link to this data.

Associated data and methods are provided in the following 5 items:

1. A readme file describing all of the associated datasets and methods for assembly and binning, as well as references.

2. An excel file (with multiple worksheets) with links to NCBI-deposited raw reads and metagenome assemblies as well as quality statistics for draft metagenome assembled genomes (MAGs).

3. A metagenome assembly created using assembler IDBA-UD v. 1.1.1 from the 2018 Illumina paired end sequencing dataset that was not deposited to NCBI as it was too similar to the assembly created with Spades v. 3.10.1 (deposited as JAUJDY010000000) but did yield a good starting bin for Thermincola.

4. FASTA files for the 79 dereplicated MAGs created from the multiple genome assemblies in different years of various subcultures of an anaerobic nitrate-reducing benzene-degrading enrichment culture (NRBC).

5. A gap-filling perl script used to assemble contigs.