Table_1.xls (5.5 kB)

Export of an Rv0132cSS–'BlaC¹ fusion protein is dependent on the Tat pathway.

dataset

posted on 2012-10-22, 01:52 authored by Ghader Bashiri, Ellen F. Perkowski, Adrian P. Turner, Meghan E. Feltcher, Miriam Braunstein, Edward N. Baker

1

'BlaC = truncated BlaC lacking its native signal sequence.

2

All strains were resistant to 20 µg/mL kanamycin due to the vector resistance marker. The presence (+) or absence (−) of carbenicillin resistance was determined by colony growth on LB–agar plates plus 20 µg/mL kanamycin and 50 µg/mL carbenicillin for M. smegmatis and 7AGT plates plus 20 µg/mL kanamycin and 50 µg/mL carbenicillin for M. tuberculosis. See materials and methods for additional experimental details.

3

Carbenicillin resistance was determined after 4–7 days.

4

Carbenicillin resistance was determined after 21 days.