Export of an Rv0132cSS–'BlaC1 fusion protein is dependent on the Tat pathway.
datasetposted on 2012-10-22, 01:52 authored by Ghader Bashiri, Ellen F. Perkowski, Adrian P. Turner, Meghan E. Feltcher, Miriam Braunstein, Edward N. Baker
'BlaC = truncated BlaC lacking its native signal sequence.2
All strains were resistant to 20 µg/mL kanamycin due to the vector resistance marker. The presence (+) or absence (−) of carbenicillin resistance was determined by colony growth on LB–agar plates plus 20 µg/mL kanamycin and 50 µg/mL carbenicillin for M. smegmatis and 7AGT plates plus 20 µg/mL kanamycin and 50 µg/mL carbenicillin for M. tuberculosis. See materials and methods for additional experimental details.3
Carbenicillin resistance was determined after 4–7 days.4
Carbenicillin resistance was determined after 21 days.