EPI-Clone dataset: Human total bone marrow (A.1-A.7)

This is the dataset supporting the EPI-Clone manuscript: Targeted single cell methylation profiling of bone marrow was performed with the scTAMseq method. BM from seven donors was analyzed Donor characteristics are described in full in Table S1.

Dataset is an R Data File (RDS) with an integrated Seurat object across donors

ASSAYS:

AB: Antibody expression data

DNAm: DNA methylation data, containing binary observations (0: amplicon not observed, i.e. dropout or absence of DNA methylation, 1: amplicon observed, i.e. DNA methylation). See the paper on scTAMseq

DIMENSIONALITY REDUCTION

scanorama: scanorama performed on the DNAm+AB data

umap: UMAP computed on scanorama

scanoprot, umapprot: Same for AB data only

scanometh, umapmeth: Same for DNAm data only

To compute donor-specific static CpGs UMAPs, follow the vignette at https://github.com/veltenlab/EPI-clone

METADATA

sample: Donor (A.1 - A.7)

age: Donor age

celltype: Cell type annotation

NonHhaI: Performance of the control amplicons in that cell

log_ChrY: Log number of reads on the ChromosomeY amplicons

EPIClone_id: Cluster annotation computed by EPI-Clone

mutation: CH mutation called in each cell. N/A means dropout.