Drosophila melanogaster wing images genetic manipulation (UAS/GAL4) of growth regulators, and sex determination genes

This will be the repository for the raw images of Drosophila melanogaster used for the analysis of wing size, wing shape and cell (trichome) density counts from a very large study manipulating key genes in both the sex determination pathway (in particular transformer, doublesex and fruitless) and genes involved with tissue growth (in particular Insulin Receptor, Target Of Rapamycin/TOR and dFoxo) among others, using the UAS/GAL4 system in Drosophila melanogaster. While the full description will be included in the readme file. Essentially all strains were (when possible) backcrossed into a common wild type strain (Samarkand, marked with white). Experiments were performed at 3 temperatures (where the flies of each genotype were reared at each temperature), 18C, 24C and 28C. Control crosses (many different control crosses were done, see details) at each temperature. This experiment was done largely using the NP6333-GAL4 (also called Pen-GAL4) but a large chunk was replicated using the nubbin-GAL4 which had stronger expression in the wing and no expression (based on GFP reporter) in the legs like NP6333-GAL4 (which made it difficult to morphological determine the sex of individuals from some crosses).