Dates of t test

This study aims to investigate the effect of fluid shear stress duration on the mechanical properties of HeLa cells, thereby elucidating the mechanical interaction between cancer cells and the fluid environment during tumor metastasis. To this end, we established an experimental system for fluid shear stress cells in vitro, and used computational fluid dynamics (CFD) software to analyze the flow field characteristics in the parallel plate flow chamber to ensure the stability of laminar flow and the uniformity of shear stress distribution. Subsequently, we used atomic force microscopy (AFM) to measure the mechanical properties of HeLa cells at different time points under physiological fluid shear stress of 10 dyn/cm². The experimental results showed that the morphology, height, and Young modulus of HeLa cells changed significantly with the increase of the duration of fluid shear stress exposure, indicating that fluid shear stress has a significant impact on the mechanical properties of cancer cells. These data provide new insights into the behavior of cancer cells in the fluid environment in vivo and provide an important theoretical basis for biomedical engineering research.

This dataset comprehensively records the effect of fluid shear stress duration on the mechanical properties of HeLa cells, aiming to reveal how different durations of fluid shear stress alter cell mechanical properties and their potential role in tumor metastasis. The dataset consists of four main components: atomic force microscopy (AFM) experimental results, computational fluid dynamics (CFD) simulation results, a 3D model of a parallel plate flow chamber system, and a statistical analysis of the mechanical properties of the battery. AFM results consisted of cell morphology images and Young's modulus plots under five experimental conditions (0, 5, 15, 30, and 60 min) at 10 dyn/cm² shear stress, analyzed using NanoScope Analysis 1.9. CFD results simulated using ANSYS Fluent 18.0 describe the flow field behavior under four shear stress conditions (5, 10, 15 and 20 dyn/cm²). The parallel plate flow chamber system designed in SOLIDWORKS 2022 includes detailed 3D models and fabrication drawings. The statistical results are presented as Excel files summarizing Young's modulus measurements of HeLa cells, providing quantitative insights into the effects of fluid shear stress on cell mechanics. Datasets can be analyzed using tools such as NanoScope 1.9 analysis for AFM data, ANSYS Fluent 18.0 for CFD results, Solidworks 2022 for 3D models, and Microsoft Excel for statistical analysis.