Date for Effects of Fluid Shear Stress Duration on the Mechanical Properties of HeLa Cells using Atomic Force Microscopy

This study investigates the effects of fluid shear stress duration on the mechanical properties of HeLa cells, aiming to elucidate the mechanical interactions between cancer cells and the fluid environment during tumor metastasis. An in vitro fluid shear stress cell experimental system was established, with flow field characteristics in a parallel plate flow chamber analyzed using computational fluid dynamics (CFD) software to ensure laminar flow stability and shear stress uniformity. Atomic force microscopy (AFM) was employed to measure the mechanical properties of HeLa cells at different time points under a physiological fluid shear stress of 10 dyn/cm². The results showed significant changes in cell morphology, height, and Young’s modulus with increasing shear stress duration, highlighting the substantial impact of fluid shear stress on cancer cell mechanics.The dataset includes AFM experimental results (cell morphology images and Young’s modulus maps under five conditions: 0, 5, 15, 30, and 60 minutes), CFD simulation results (flow field characteristics under four shear stress conditions: 5, 10, 15, and 20 dyn/cm²), 3D models of the parallel-plate flow chamber system, and statistical analyses of cell mechanical properties. These data provide new insights into cancer cell behavior in the fluid environment and offer important theoretical foundations for biomedical engineering research. The dataset can be analyzed using tools such as NanoScope Analysis 1.9 for AFM data, ANSYS Fluent 18.0 for CFD results, Solidworks 2022 for 3D models, and Microsoft Excel for statistical analysis.