Dataset RT-qPCR

Name: Dataset RT-qPCR
Published: 2025-04-15T15:11:46+00:00
License: https://creativecommons.org/licenses/by/4.0/
Keywords: RT-qPCR expression levels

dataset

posted on 2025-04-15, 15:11 authored by Iker MartinezIker Martinez

The enclosed data includes RT-qPCR data

We employed CRISPR activation of the gene TSG-6 in MSCs to evaluate the impact of elevated TSG-6 on EV cargo and immunomodulatory function in an in vitro macrophage model. CRISPR-mediated gene activation was confirmed by RT-qPCR.

Macrophages were co-treated with LPS and TSG-6-overexpressing MSC-EVs, and gene expression of pro-inflammatory cytokines was measured by RT-qPCR.