Data from: Colonial choanoflagellate isolated from Mono Lake harbors a bacterial community

As the closest living relatives of animals, choanoflagellates may offer insights into the ancestry of interactions among animals and bacteria. Here we report the isolation and characterization of a colonial choanoflagellate species, Barroeca monosierra harbors a bacterial community in its lumen. B. monosierra was isolated from Mono Lake, California and forms large spherical colonies that are an order of magnitude larger than those formed by the closely related choanoflagellate Salpingoeca rosetta. The rosettes, which can develop from single cells, are filled with a branched network of undefined extracellular matrix. We found that lab-reared B. monosierra colonies are colonized by members of the halotolerant Saccharospirillaceae and Oceanospirillaceae, as well as purple sulfur bacteria (Ectothiorhodospiraceae) and non-sulfur Rhodobacteraceae. This relatively simple bacterial community in a close relative of animals presents a new experimental model for investigating the evolution of stable interactions among eukaryotes and bacteria.

This dataset contains the genome sequence of B. monosierra, as well as 24 associated bacterial genome sequences.

The two ZIP archives whose names begin with "tree" contain all relevant data from the phylogenetic trees presented in Figure 1C and Figure S1. Each archive contains the following subdirectories:

input: input sequences in FASTA format

polyA_removed: polyA tails removed by trimest (for protein coding genes only)

aligned: sequence alignments by FSA

trimmed: trimmed alignments by trimAl

RAxML: input, run parameters and output of RAxML maximum likelihood tree reconstruction

MrBayes: input, run parameters and output of MrBayes Bayesian tree reconstruction

The file Figure_2E.tree contains the tree from Figure 2E in Newick format.