Data from: A large colonial choanoflagellate from Mono Lake harbors live bacteria

As the closest living relatives of animals, choanoflagellates offer insights into the ancestry of animal cell physiology. Here, we report the isolation and characterization of a colonial choanoflagellate from Mono Lake, California. The choanoflagellate forms large spherical colonies that are an order of magnitude larger than those formed by the closely related choanoflagellate Salpingoeca rosetta. In cultures maintained in the laboratory, the lumen of the spherical colony is filled with a branched network of extracellular matrix and colonized by bacteria, including diverse Gammaproteobacteria and Alphaproteobacteria. We propose to erect Barroeca monosierra gen. nov., sp. nov. Hake, Burkhardt, Richter, and King to accommodate this extremophile choanoflagellate. The physical association between bacteria and B. monosierra in culture presents a new experimental model for investigating interactions among bacteria and eukaryotes. Future work will investigate the nature of these interactions in wild populations and the mechanisms underpinning the colonization of B. monosierra spheres by bacteria.

This dataset contains the genome sequence of B. monosierra (NKKH001C_Barroeca_monosierra.fa.gz), as well as 24 associated bacterial genome sequences (bacterial_genomes.zip).

The two ZIP archives whose names begin with "tree" contain all relevant data from the phylogenetic trees presented in Figure 1C and Figure S1. Each archive contains the following subdirectories:

input: input sequences in FASTA format

polyA_removed: polyA tails removed by trimest (for protein coding genes only)

aligned: sequence alignments by FSA

trimmed: trimmed alignments by trimAl

RAxML: input, run parameters and output of RAxML maximum likelihood tree reconstruction

MrBayes: input, run parameters and output of MrBayes Bayesian tree reconstruction

The file Figure_2E.tree contains the tree from Figure 2E in Newick format.