DATA FRAME-DROSOPHILA MELANOGASTER - AYAHUACA - SUCROSE.xlsx

The study aimed to investigate the impact of Ayahuasca, a classic psychedelic, on cognition and sucrose consumption patterns in Drosophila melanogaster. This was done considering the common pathways between these substances and the neurological alterations caused by these foods. Using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), we investigated the concentrations of the active principles dimethyltryptamine, harmaline, harmine, and tetrahydroharmaline present in Ayahuasca, which were 221.62 µg/ml, 26.33 µg/ml, 769.01 µg/ml, and 164.78 µg/ml, respectively. The toxicity of Ayahuasca was assessed through exposure tests, involving the calculation of survival rates using Ayahuasca administered in the diet at concentrations of 100%, 75%, 50%, 25%, or 5%. Subsequently, cognitive performance was evaluated using Open Field and Negative Geotaxis assays. After toxicity testing, the flies were previously sensitized through modified diets, one enriched with sucrose and another supplemented with the dopaminergic precursor L-DOPA. Then, sucrose solution consumption was measured using the Capillary Feeding assay (CAFÉ), with this process occurring after exposure to Ayahuasca at 5% and 50% concentrations for a portion of each group.d