Costa et al. Multiple mating rescues offspring sex ratio but not productivity in a haplodiploid exposed to developmental heat stress
Summary:
The data and scripts presented were used to assess how a sublethal high temperature (36°C) affects the reproductive system of a haplodiploid species. Using spider mites (Tetranychus urticae) developed either at 25°C or 36°C, we paired individuals in four treatments (♀25°C x ♂25°C, ♀25°C x ♂36°C, ♀36°C x ♂25°C, ♀36°C x ♂36°C), to measure the effect of temperature on the fertility of both sexes, mating and remating eagerness, and the paternity of the offspring of females with different mating histories.
Files available:
We provide 3 .R files (Script_statistical_analysis_Fertility_and_Paternity_Share, Script_statistical_analysis_Mating_Behavior, Script_figures) and 6 .csv files (Dataset_Fertility_and_Paternity_share, Dataset_altered_Fertility_and_Paternity_share, Dataset_means_Fertility_and_Paternity_share, Dataset_Mating_behavior, Dataset_means1_Mating_behavior, Dataset_means2_Mating_behavior).
Script_statistical_analysis_Fertility_and_Paternity_Share
This file contains the script used to make the statistical analysis for the Fertility and Paternity Share experiment.
Script_statistical_analysis_Mating_Behavior
This file contains the script used to make the statistical analysis for the Mating Behavior experiment.
Script_figures
This file contains the script used to create all the figures.
Dataset_Fertility_and_Paternity_share
This file contains the raw data of the effect of temperature on fertility and paternity share.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the male (25°C or 36°C).
Type_M - mating history of the female (SM, if single mated; DM, if double mated; MM, if multiple mated).
Rep - replicate number per treatment.
Block - day in which matings were observed.
Rtemp - temperature of the observation room.
Rhum - humidity of the observation room.
Fecundity - number of eggs laid during 4 days per female.
Unhatched_eggs - number of eggs that did not develop.
Total_daughters - number of adult daughters counted.
Total_sons - number of adult sons counted.
Total_contribution_males - sum of adult daughters and unhatched eggs.
Total_offspring - sum of adult daughters, adult sons and unhatched eggs.
Prop_daughters_ue - proportion of adult daughters in the fertilized offspring (Total_daughters / Total_contribution_males).
Prop_fert_off - proportion of adult daughters and unhatched eggs in the offspring (Total_contribution_males / Total_offspring).
Observations - any observations made in each replicate.
Dataset_altered_Fertility_and_Paternity_share
This file contains the same raw data as Dataset_Fertility_and_Paternity_share, and includes 4 more lines (in SM from each treatment) to deal with the lack of variance in Total_daughters and Unhatched_eggs, which impeded the statistical analysis.
Table headers are similar to the ones in Dataset_Fertility_and_Paternity_share.
Dataset_means_Fertility_and_Paternity_share
This file contains the means of each variable studied for the effect of temperature on fertility and paternity share.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the first male (25°C or 36°C).
Type_M - mating history of the female (SM, if single mated; DM, if double mated; MM, if multiple mated).
Mean_prop_fem_ue - mean proportion of daughters and unhatched eggs in the offspring per treatment and female mating history.
Mean_total_off - mean number of offspring (daughters, sons and unhatched eggs) per treatment and female mating history.
Mean_prop_fem - mean proportion of adult daughters in the fertilized offspring (daughters and unhatched eggs) per treatment and female mating history.
Dataset_Mating_behavior
This file contains the raw data of the effect of temperature on mating behavior.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the male (25°C or 36°C).
Type_M - mating history of the female (SM, if single mated; MM, if multiple mated).
Rep - replicate number per treatment.
Block - day in which matings were observed.
Rtemp - temperature of the observation room.
Rhum - humidity of the observation room.
Mating_rate - number of times the female mated.
Lat_sec1 - latency to the first copulation of the female.
CopYN - if the female mated a first time (1=yes, 0=no).
Cop_sec1 - duration of the first copulation of the female.
Lat_sec2 - latency to the second copulation of the female.
Cop2YN - if the female mated a second time (1=yes, 0=no).
Cop_sec2 - duration of the second copulation of the female.
Fecundity - number of eggs laid during 4 days per female.
Total_daughters - number of adult daughters counted.
Total_sons - number of adult sons counted.
Sex_ratio - proportion of adult daughters in the offspring (adult daughters and sons).
Total_adults - sum of adult daughters and sons.
Observations - any observations made in each replicate.
Dataset_means1_Mating_behavior
This file contains the means of each variable studied for the effect of temperature on mating behavior.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the first male (25°C or 36°C).
Mean_lat1 - mean latency to the first copulation of the female per treatment.
Mean_cop1 - mean duration of the first copulation of the female per treatment.
Mean_lat2 - mean latency to the second copulation of the female per treatment.
Mean_cop2 - mean duration of the second copulation of the female per treatment.
Dataset_means2_Mating_behavior
This file contains the means of each variable studied for the effect of temperature on mating behavior present in the supplementary material.
Table headers are explained below:
Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).
Temp_fem - developmental temperature of the female (25°C or 36°C).
Temp_male - developmental temperature of the first male (25°C or 36°C).
Type_M - mating history of the female (SM, if single mated; MM, if multiple mated).
Mean_off - mean number of offspring (adult daughters and sons) per treatment and female mating history.
Mean_sexratio - mean proportion of adult daughters in the offspring (adult daughters and sons) per treatment and female mating history.
