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Canine fecal samples

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posted on 2017-10-18, 16:30 authored by Guillaume JospinGuillaume Jospin
To determine the optimal method for fecal DNA preservation, we collected fecal samples from one canine donor and stored aliquots in RNAlater, 70% ethanol, 50:50 glycerol:PBS, or without buffer at 25°C, 4°C, and -80°C. Fecal DNA was extracted, quantified, and 16S rRNA gene analysis performed on days 0, 7, 14, and 56 to evaluate changes in DNA concentration, purity, and microbial diversity and composition over time

Funding

"Matching Funds Award" (Grant ID CCAH 2015-38-F/M, University of California, Davis Center for Companion Animal Health) "Novel use of Fecal Microbiota Transplantation (FMT) in Dogs with Non-Seasonal Atopic Dermatitis" (Grant ID OVCR-201503617, Nestle Purina PetCare Company)

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