Astec-Pm6 (Wild type Phallusia mammillata embryo, live SPIM imaging, stages 7-13)

dataset

posted on 2020-07-09, 21:59 authored by Patrick LemairePatrick Lemaire

Astec-Pm6 Live embryonic development of Phallusia mammillata (Phlebobranch ascidian).

Cell membranes were fluorescently labeled by microinjection of mRNA encoding PH-citrine into unfertilized egg. The embryo has been live imaged with a MuVi-SPIM for three hours, from the 44-cell stage (Tp 1, St. 7) up to the late gastrula (Tp 88, St. 13), at 18°C.

Whole cells were segmented using the ASTEC pipeline. Surface meshes were produced using the VTK library and MeshLab.

The period between time points is 115 seconds after normalization on the average development at 18°C.

* The four angle fusion images obtained using the ASTEC pipeline are shared in .inr format (fuse.tar.gz archive file).

* Whole cells were segmented using the ASTEC pipeline and shared in .inr format (post.tar.gz archive file).

* Surface meshes (.obj) povided in the mesh.tar.gz archive were produced using the VTK library and MeshLab.

* Individual cell geometric properties are shared in two different formats, .xml and .pkl (properties.tar.gz archive file).

Funding

CNRS, INRIA, Agence Nationale de la Recherche, Fondation Schueler Bettencourt, Fondation pour la Recherche Médicale

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Keywords

light-sheet microscopes ascidian Phallusia mammillata ascidian development phallusia mammillata muvispim ascidian embryos Developmental Biology Cell Development, Proliferation and Death Animal Developmental and Reproductive Biology Computational Biology Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)

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CC BY 4.0

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