impala-blood.zip (4.44 MB)

Analysis scripts and supplementary files: Blood colour can be used to assess arterial blood oxygenation in immobilised impala (Aepyceros melampus)

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posted on 01.06.2020, 08:52 by Etienne Basson, Gareth Zeiler, Peter Kamerman, Leith Meyer
Background To determine the relationship between arterial blood colour (CIE L*a*b*) and blood oxygenation [functional oxyhaemoglobin saturation (SaO2) and fractional oxyhaemoglobin saturation (FO2Hb)], and if arterial blood colour can be used to predict arterial blood oxygenation.

Methods A prospective randomised crossover study where 11 female impala were immobilised with potent opioids. Arterial blood was collected anaerobically into heparinised syringes and analysed immediately using spectrocolourimetry and co-oximetry. Data were analysed by modelling the relationship between predicted CIE L*a*b* components and SaO2 and FO2Hb, and assessing the subjective ordering of the resulting colour palette by 20 observers using a modified version of the Farnsworth-Munsell hue test.

Results The second-order polynomial (quadratic) model produced the best fit for all three CIE L*a*b* components for SaO2 and FO2Hb. The regression models were used to generate predicted CIE L*a*b* co-ordinates over a range of SaO2 and FO2Hb percentages (15 to 95%). The resulting colour palettes were correctly ordered by all observers, in the SaO2 and FO2Hb ranges of 45 to 95% saturation.

Conclusions An association between the blood colour components (CIE L*a*b*) and SaO2 and FO2Hb exists and arterial blood colour can be used to reliably estimate arterial blood oxygenation of impala.

Funding

Department of Paraclinical Sciences, Faculty of Veterinary Sciences, University of Pretoria

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