Amplicon Sequencing Data for Bacteria (16S) and Fungi (ITS) (Raw)

<p dir="ltr">Raw sequencing dataset from an experimentally simulated a short-term precipitation pulse (50 mm) in soils from the hyperarid Atacama Desert to evaluate shifts in microbial community composition, diversity, and functional potential, using a microcosm experiment. DNA extraction was performed using the DNeasy® PowerSoil® Pro Kit (Qiagen), following the manufacturer’s protocol, and DNA concentration was determined using a Qubit™ 4.0 fluorimeter (ThermoFisher Scientific). The 16S ribosomal RNA gene, tar-geting the V3-V4 region, and the ITS ribosomal RNA were sequenced at Zymo Research. Primers for sequencing were provided by the same company, and sequencing was performed using the Illumina® MiSeq™ platform. Zymo Research conducted the taxonomic assignment of fungi and bacteria using UCLUST in QIIME v.1.9.1, referencing their proprietary database. The absolute abundance of Amplicon Sequence Variants (ASVs) was determined by Zymo Research using qPCR, with a standard curve for both the 16S and ITS genes.</p>