All demultiplexed sequence data from aDNA, raw ASV tables and metadata from wDNA and aDNA

1. CO1_ASV_taxonomy_detailed.txt

This file contains all Amplicon Sequence Variants (ASVs) recovered from sequence data processed through the Anacapa pipeline, corresponding to the taxonomy_detailed.txt output files generated by Anacapa. The dataset includes reads from both water samples and ARMS (Autonomous Reef Monitoring Structures) samples. For each ASV, the following details are provided:

• DNA sequence
• Read abundance per sample
• Blast alignment statistics
• Assigned taxonomy
• Taxonomy confidence score (BCLA)
• Accession numbers of blast matches

1. df_table.txt

This file provides contextual information for each sample, including the following columns:

• sample (corresponding metabarcode for each sequence file)
• sample_name (abreviatura del sample nmame)
• Site (collection location)
• ocean_depth
• fraction_size (for ARMS samples; indicates size fraction category: sessile, 2000-500 μm [mobile_500], and 500-100 μm [mobile_100])
• replicate (replicate identifier)
• sample_media (sample type: ARMS or water)
• season (for water samples; e.g., summer, winter)
• sample_type (categorization as sample or control, with control type specified if applicable)

1. demutiplexed_arms (2)
   The dataset contains compressed (zip) files with 108 paired FASTQ files (R1 and R2), representing demultiplexed ARMS eDNA samples sequenced for COI. Files follow a structured naming convention indicating sample attributes. Complete sample information is available in the metadata file (df_table.txt).