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A gene expression atlas of a bicoid-depleted Drosophila embryo

dataset
posted on 23.12.2014, 14:53 authored by Max StallerMax Staller, Charless C. Fowlkes, Meghan D. J. Bragdon, Zeba Wunderlich, Javier Estrada, Angela H. DePace, Tara Lydiard-Martin

Data accompanying Staller et al. Development, in press (open access link will be included when available) and used in Staller et al., PNAS, in press (open access link will be included when available). These data were generated in the DePace Lab at Harvard Medical School, using protocols developed by the Berkeley Drosophila Transcription Network Project (BDTNP) and the DePace Lab. Files include:

bcdrnai_20140807_v1.1.vpc : a cellular resolution gene expression atlas of an average Drosophila melanogaster embryo depleted of the maternal transcription factor bicoid. Contains expression patterns for 13 genes (caudal (cad), Krüppel (Kr), knirps (kni), giant (gt), hunchback (hb), fork head ( fkh), huckebein (hkb), tailless (tll), Dichaete (D), runt (run), hairy (h), even-skipped (eve) and fushi-tarazu ( ftz)) and 6 enhancer reporter constructs (hb posterior, gt posterior, eve stripe3+7, eve stripe5, two eve stripe4+6 enhancers, and a whole locus eve reporter).

Dmel_reporters_20141211.vpc : a cellular resolution gene expression atlas of eve enhancer reporter constructs in wild type Drosophila melanogaster embryos. The enhancers are: (eve stripe1, eve stripe2, eve stripe2+7 eve stripe3+7, eve stripe5, two eve stripe4+6 enhancers, and a whole locus eve reporter).

Deml_snahb_20141223.vpc : a cellular gene expression atlas of eve enhancer reporters in embryos carrying 2 copies of an activated snail::hunchback transgene.

EveFtzPCS_forDistributions.zip : individual point cloud files for embryos co-stained for even-skipped (eve) and fushi tarazu (ftz). Data from additional genes is available upon request.

Data can be analyzed using the BDTNP PointCloudToolBox and visualized using the BDTNP PointCloudXplore program which are both freely available (links below). Please contact the DePace lab with any additional questions (link below).

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