AMOVA and pairwise alignment Input files

These files in format (.arp) were used to run an AMOVA analysis in Arlequin 3.5. These files have been modified from existing files from Kamath et al. 2016 to include New Mexico sequences and other Rana catesbeiana cyt b sequences on Genbank. AMOVA compared each region of invasive and native populations to New Mexico populations.

(.arp) files are directly input to Arlequin software. Files contain haplotypes and sample sizes for each region in order.(.hap) files are contained in same folder and include sequences and associated haplotype.

Settings in Arlequin:
AMOVA: Locus by Locus AMOVA, 10,000 permutations, compute distance matrix by pairwise differences

Population comparisons: Compute pairwise FST, 10,000 permutations, significance level 0.05, compute distance matrix by pairwise differences