3k PBMCs from a healthy donor

This dataset contains single-cell RNA sequencing (scRNA-seq) data of 3,000 peripheral blood mononuclear cells (PBMCs) from a healthy donor, processed using the 10x Genomics Chromium platform. The raw data was obtained from 10x Genomics and subsequently aligned using Cell Ranger 8.0.1 with the GENCODE Release 47 (GRCh38.p14) reference genome.

The dataset includes the following output files from the Cell Ranger pipeline:

• filtered_feature_bc_matrix.h5 – Filtered count matrix in HDF5 format
• filtered_feature_bc_matrix – Filtered gene-barcode matrix in directory format
• raw_feature_bc_matrix – Raw gene-barcode matrix in directory format
• raw_feature_bc_matrix.h5 – Raw count matrix in HDF5 format

This dataset is valuable for researchers studying single-cell transcriptomics, immune cell profiling, and bioinformatics pipeline benchmarking.

File format: HDF5 and Matrix Market (MTX)
Reference Genome: GENCODE Release 47 (GRCh38.p14)
Processing Pipeline: Cell Ranger 8.0.1

For any questions or collaborations, please feel free to contact the uploader.