27f/1392r sheared soil amplicons for EMIRGE
We
extracted DNA from 6 soils and
used the 27f/1392r primer pair to amplify near full-length 16S rRNA
genes.
The amplicons were sheared using the Covaris M220 (Covaris, Woburn, MA) and gene libraries were prepared using
TruSeq DNA LT library preparation kits (Illumina, San Diego, CA). Samples were
pooled and sequenced on an Illumina MiSeq (2x300bp) at the University of
Colorado Next Generation Sequencing Facility.
We used these raw sequences to re-assemble the near full length 16S rRNA amplicon using EMIRGE.
