1) FCS files from spectral flow cytometry experiments performed on AML patient blasts. 2) HDR donor for pSTAT1 to add the degron tag and mCherry expression.

<p dir="ltr">From methods section of the article:<br></p><p dir="ltr">AML patient blast samples were obtained from the Vanderbilt University Medical Center Hematology Tissue Repository. One portion containing 400,000 cells was split into two conditions: 1) PBS control and 2) IFNγ-stimulated for 15 minutes. After stimulation, cells were fixed in 1.6% PFA and permeabilized in ice-cold methanol. Barcoding was performed using Pacific Orange (PO) (ThermoFisher, P30253) at two distinct concentrations (high: 100 ng/mL, low: 12 ng/mL). The barcoded cells were washed, pooled, and stained with an antibody cocktail (table S7). Spectral flow cytometry was conducted using the Cytek Aurora (Cytek Biosciences) to measure key surface marker expression and STAT1 phosphorylation.<br><br>A cloning plasmid (IDT, pUCIDT-AMP) was modified to serve as the homology-directed repair (HDR) donor by incorporating the STAT1 sequence from the cut site to the stop codon, an FKBP12F36V degron tag, an HA-tag, a P2A sequence, and an mCherry fluorescent protein sequence. These elements were flanked by a 5′ homology arm (200 bp) and a 3′ homology arm (450 bp). The HDR donor plasmid map is available on Figshare.</p><p><br></p>