figshare
Browse

16S rRNA amplicon sequencing of dauciform root, rhizosphere, and bulk soil communities of <i>Cladium jamaicense</i> in the Florida Everglades

dataset
posted on 2025-10-06, 01:15 authored by Kevin MontielKevin Montiel, John S. Kominoski, Anna K. Simonsen
<p dir="ltr">This dataset contains raw 16S rRNA gene amplicon sequencing reads from bulk soil, rhizosphere soil, and dauciform root rhizosphere samples of <i>Cladium jamaicense</i> (sawgrass) collected in July 2024 from calcareous (marl) soils near Everglades National Park, Florida, USA. <i>C. jamaicense</i> produces specialized dauciform roots that release carboxylates to mobilize bound phosphorus in nutrient-limited environments, such as the extremely phosphorus-poor wetland soils of the Everglades.</p><p dir="ltr">Genomic DNA was extracted using the DNeasy PowerSoil Kit (QIAGEN) following the manufacturer’s instructions. The 16S rRNA gene was amplified using the primer pair 341F-805R and sequenced on an Illumina NextSeq 2000 platform with a NextSeq 300 PE P1 flow cell at the North Carolina State University Genomic Sciences Laboratory. Raw paired-end Illumina reads generated by the sequencing facility are deposited in this submission.</p>

History