This is the singularity version of 16S amplicon sequences analysis pipeline from UNSW Microbiome Research Centre, the pipeline process raw pair-end Miseq sequences to do quality control using DADA2 integreated into QIIME2, performing core diversity analysis using QIIME2 plugin and integrate LEfSe for a simple differential abundance analysis for case and control group analysis.
The tutorial of how to use this singularity version of the pipeline is at: https://github.com/biofuture/microbiome_singulairty_version
The initial aim of integrating the pipeline into Singularity is to streamline the analysis and avoid installation of third parity bioinformatics software by users. Users can use the tool directly with a uniform singularity interface.