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Effect of survivin on senescence.

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posted on 19.01.2012 by Caterina A. M. La Porta, Stefano Zapperi, James P. Sethna

IGR37 ABCG2− cells were grown until the peak of (98 days see Table S1) and then transfected with a plasmid containing cytoplasmic-survivin-GFP or GFP alone. The next day the cells were plated for activity ( well) and crystal violet (200 cells per well) assays. In panel A, we show the percentage of positive cells for untransfected, GFP and GFP-survivin cells. The histogram shows the mean of five independent determination S.E. Represenative images of staining (shown in green) are reported for GFP and GFP-survivin cells. In panel B, we show the distribution of colony sizes from crystal violet assay for GFP and GFP-survivin cells, showing a marked increase in proliferation. Such an increase is not detected for unsorted IGR37 cells, transfected at the beginning of the experiment, for which the percentage of positive cells is already low.

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