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Fluorescence and bright field images of test and control algae and Daphnia.

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posted on 20.02.2013 by Tommy Cedervall, Lars-Anders Hansson, Mercy Lard, Birgitta Frohm, Sara Linse

A. Bright field images of algae taken from test sample, After 24 h incubation with 28 nm fluorescently labeled nanoparticles. B. Fluorescence micrograph of algae from A, imaged with Deep Blue filter cube (see Methods), here algal cells are fluorescent due to adsorption of fluorescently labeled nanoparticles. C. Bright field images of algae taken from control sample. D. Fluorescence micrograph of algae from C, imaged with Deep Blue filter cube, here algal cells are clearly non-fluorescent. Scale bars, A–D: 10 µm. E. Fluorescence micrograph of Daphnia, taken after 24 hrs incubation with test algae and a light wash through filter. F. Close up of E, nanoparticles can be seen in and on the Daphnia. G. Fluorescence micrograph of control Daphnia, taken after 24 hrs incubation with control algae and a light wash through filter. H. Close up of G, note some auto-fluorescence in the gut and heart and developing offspring, much of which is distributed evenly. Scale bars, E–H: 500 µm.

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