Fig_5.tif (1.01 MB)
trkANGF expression in isolated untreated and NGF-treated photoreceptors.
figure
posted on 2015-04-21, 03:02 authored by Maria Luisa Rocco, Bijorn Omar Balzamino, Pamela Petrocchi Passeri, Alessandra Micera, Luigi AloePhotoreceptors were isolated from p10 retinal cells that were cultured alone (untreated) or stimulated with NGF or neutralized (ANA) for 6 consecutive days. (A) Photoreceptor isolation was performed as described in M&M. (B) Representative side scatter (FSC/SSC) showing the Rhodopsin backgating over a total retinal cell population (10000 events). (C-D) TrkANGFR protein (C) and target gene (D) expression in photoreceptors isolated from cultures exposed to NGF (50ng/mL) or ANA (500ng/mL). Note the increased trkANGFR expression upon NGF exposure. (DAPI nuclear staining; Magnifications: A,B: x400; C-H; x600/oil immersion).