figshare
Browse
Figure_3.tif (281.07 kB)

siRNA targeting NRF1 mRNA knocks down SPAST

Download (0 kB)
figure
posted on 2012-05-04, 00:54 authored by Brian J. Henson, Wan Zhu, Kelsey Hardaway, Jaime L. Wetzel, Mihaela Stefan, Kathryn M. Albers, Robert D. Nicholls

mRNA expression. SK-N-SH cells were transfected with a pSUPER-NRF1 shRNA expression vector or mock transfected, with qualitative analysis of gene expression including a negative control (GAPDH). The quantitative band intensities for the “siRNA” and “mock” transfection samples were compared and the ratio is listed to the right of the gel images. The GAPDH mRNA level is unaffected by siRNA targeting NRF1, whereas the mRNA levels for NRF1 and SPAST are reduced by the siRNA treatment. The experiment was repeated three times with equivalent results to the representative results shown here.

History

Usage metrics

    PLOS ONE

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC