Figure_13.tif (4.04 MB)

siRNA against p21 made ISA27 unable to induce ΔΨm dissipation and PE.

figure

posted on 2013-08-19, 03:55 authored by Barbara Costa, Sara Bendinelli, Pamela Gabelloni, Eleonora Da Pozzo, Simona Daniele, Fabrizio Scatena, Renato Vanacore, Pietro Campiglia, Alessia Bertamino, Isabel Gomez-Monterrey, Daniela Sorriento, Carmine Del Giudice, Guido Iaccarino, Ettore Novellino, Claudia Martini

Random siRNA and p21siRNA samples were exposed to a fixed dose of ISA27 (5 µM) or DMSO for 24 h. A) Evaluation of ISA27 effect on the ΔΨm in p21 siRNA samples. The ISA27-treated random siRNA sample showed dissipation of ΔΨm compared to random siRNA sample. The comparison of ΔΨm between p21 siRNA and ISA27-treated p21 siRNA sample did not show statistically significant differences. Upper panel shows representative dot plots of untreated and ISA27-treated random or p21siRNA samples. B) Evaluation of ISA27 effect on PE in p21 siRNA samples. The ISA27-treated random siRNA sample showed a statistical significant increase of early and late apoptosis compared to random siRNA sample. The comparison of early and late apoptosis between p21 siRNA and ISA27-treated p21 siRNA sample did not show statistically significant differences.