siRNA against p21 made ISA27 unable to induce ΔΨm dissipation and PE.

<p>Random siRNA and p21siRNA samples were exposed to a fixed dose of ISA27 (5 µM) or DMSO for 24 h. A) <i>Evaluation of ISA27 effect on the ΔΨm in p21 siRNA samples</i>. The ISA27-treated random siRNA sample showed dissipation of ΔΨm compared to random siRNA sample. The comparison of ΔΨm between p21 siRNA and ISA27-treated p21 siRNA sample did not show statistically significant differences. Upper panel shows representative dot plots of untreated and ISA27-treated random or p21siRNA samples. <i>B) Evaluation of ISA27 effect on PE in p21 siRNA samples.</i> The ISA27-treated random siRNA sample showed a statistical significant increase of early and late apoptosis compared to random siRNA sample. The comparison of early and late apoptosis between p21 siRNA and ISA27-treated p21 siRNA sample did not show statistically significant differences.</p>