siRNA against α-SMA inhibits both basal and EGF-induced cell migration in adult fibroblasts.

Cells were incubated in the presence or absence of EGF(1 nM) +/− siRNA against α-SMA in an in vitro wound healing assay. In all experiments a non-specific control siRNA was used as a control. Cell motility is displayed as a relative percentage of baseline motility in the absence of EGF or siRNA exposure (100%). Active siRNA versus α-SMA reduced both basal and EGF-induced motility; a non-specific control siRNA had no such effect. Values are means ± SEM of eight independent studies, each performed in duplicate. Statistical analyses were performed with Student's t test.