qRT-PCR on EE and ST retinal samples.

A–C Relative amounts of mRNA for NGF, BDNF and IGF-1 detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Gray bars refer to age matched wild type mice also kept in ST. For all the assays, Rq values (referred to GADPH mRNA) in EE were normalized to those in ST. One way Anova with Bonferroni post-hoc analysis. Columns show average values +/− SEM. It is to note that the decrease of NGF observed in EE rd10 mice at 45 days of age (p = 0.006) is only with respect to ST controls but not in comparison to age-matched wild type mice. D. Relative amounts of mRNA for CNTF and mTOR detected by qRT-PCR in retinal extracts from rd10 mice raised in ST (black bars) and EE (red bars) at 3 different ages. Extracts were from groups of 8–10 retinas for each age. Columns show average values +/− SEM. Statistic differences calculated by one-way Anova with Bonferroni post-hoc analysis are given in the text.