puc is required in neurons for ethanol-induced locomotor stimulation.

A, Quantitative PCR detection of puc expression levels in fly head extracts following 30 min ethanol or air control exposure. **p = 0.0024 t test. B, Reduced ethanol-induced locomotor stimulation in puc¹/+ flies. C, Quantification of traces shown in B. ***p<0.0001 t test. Ethanol absorption of puc1/+ was unaffected (p = 0.9607 t test). D, Mushroom body morphology of puc¹/+ mutants was normal. Scale bar = 10 µm. E, Decreased ethanol-induced locomotor stimulation of puc¹/+ mutants rescued by neuron-specific expression of puc. puc¹/+ mutants carrying a UAS-puc transgene were crossed to the elav^C155-Gal4 to restore puc expression levels specifically in neurons. F, elav^C155-Gal4 did not increase ethanol-induced locomotor stimulation in puc¹/+ mutants.