miR-33b is downregulated in osteosarcoma.

(A) The expression relative expression levels were determined by qRT-PCR in human osteoblast cell line (hFOB) and osteosarcoma cells (SOSP-9607, U2OS, MG-63, SAOS-2). (B) qRT-PCR analysis of miR-33b expression in 60 pairs osteosarcoma tissues and their corresponding adjacent normal bone tissues. The expression of miR-33b was normalized to U6 snRNA. (C) Relative miR-33b expressionlevels in osteosarcoma tissues and their corresponding adjacent normal bone tissues. (D) Association between miRNA level between pM stage (No metastasis and Metastasis). Student’s t test was used to analyze the significant differences between the tumor and normal tissues. Correlation of miR-33b expression with patients’clinicopathological variables was evaluated by one-way analysis of variance test. ***p<0.001.