mRNA expression levels of LITAF in hemocyte monolayers.

Samples were collected at 0, 1.5, 4, 8 and 12 h after RLO (1ul/106 cells) + anti-CaHMGB (1∶1000) and LPS (100 ng/ml) + anti-CaHMGB (1∶1000) incubation. Expression levels were assessed by real-time RT-PCR and seawater-incubated groups were used as controls. Expression levels were assessed using 28SrDNA gene for normalization. *p≤0.05 when compared to control value. Δ p≤0.05 when RLO/LPS+anti-CaHMGB group value compared to RLO/LPS group value. (A) RLO+ anti-CaHMGB; (B) LPS+anti-CaHMGB.