X. pisi apiose operon and phylogeny of GTs.

(A) Gene organization of the apiose operon in X. pisi genome (NZ_JPLE01000032.1) from position 177,470 to 181,515 bp. -35 and -10 promoters were predicted using Bacterial Promoter Prediction website (BacPP, https://molbiol-tools.ca/Promoters.htm). Operon schema was generated using SnapGene Viewer version 3.1.4 (GSL Biotech, Chicago, IL). (B) phylogenetic analysis of XpApiT (PPU69163) and XpXylT (PPU69164). Amino acid sequences from representatives of pfam05686 and pfam13704 selected by the CDD were used. Alignments were made using Clustal Omega [23–25] and the trees generated using Dendroscope [26]. Distance scale represents difference between sequences in substitutions per site. For full amino acid sequence alignment with organism and gene names see S1 and S2 Figs.