<i>Wolbachia</i> density and distribution in transinfected <i>Ae</i>. <i>aegypti</i> lines.

<p>(A) Density of <i>Wolbachia</i> within 5-, 10- and 15-day old whole female mosquitoes was determined by qPCR using primers directed to the conserved <i>16S</i> rRNA gene. Density is expressed as the mean ratio between <i>16S</i> and the <i>Ae</i>. <i>aegypti</i> host <i>rps17 gene</i>. Data are the mean and SEM of 24 mosquitoes. Asterisks indicate significance compared to <i>w</i>Mel at each time point (Kruskal-Wallis, Dunn's test with multiple test corrections; *p<0.05, **p<0.01, ***p<0.001, n.s. not significant). (B) The distribution of <i>w</i>MelCS, <i>w</i>Ri and <i>w</i>Pip <i>Wolbachia</i> strains in mosquitoes was determined in sections of paraffin-embedded female mosquitoes (5 to 7-day old) using fluorescence <i>in situ</i> hybridisation (FISH). The fluorescently labelled 16S probe detects the <i>16S</i> rRNA gene from all four <i>Wolbachia</i> strains. Total DNA was stained in blue using DAPI and a green filter was included to increase contrast with surrounding tissues. <i>Sg</i> indicates salivary gland tissue, <i>m</i> indicates muscle, and <i>c</i> indicates cardia. White arrows identify select regions of <i>Wolbachia</i> staining.</p>