Tsc1 FoxO double mutant discs exhibit misfoldings due to massive overproliferation under NR.

(A) aPKC (in green), phalloidin (in red) and DAPI (in blue) stainings in orthogonal sections of eye discs with control, FoxO, Tsc1 and Tsc1 FoxO mutant tissue dissected from larvae reared on normal food and NR (A’). Scale bars are 25 μm except for Tsc1^Q87X FoxO²⁵ on NR, where they are 75 μm. A lower magnification was used to give a better overview of the misfolded tissue. (B) aPKC (in green) and Dlg (in red) stainings in orthogonal sections of eye discs with hsFlp control, FoxO, Tsc1, and Tsc1 FoxO mutant clones (marked by the absence of GFP) dissected from larvae reared on normal food and NR.